Hotspot

rpl20-rps12

Genome · 63598-64550 · 953 bp

Back to Goniophlebium

Pi 0.0973

MarkerSeek score 81.80

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: Genome
length_bp: 953
variable_sites: 91
pi: 0.0973
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCTCGTTTCACTCTGGTCA	AGGGGTTTGTACTAGGGTGT	50.0	50.0	58.3	57.6	979-996 bp	1.0	83.0
rpl20-rps12_p2	GGATCCTCGTTTCACTCTGG	AGGGGTTTGTACTAGGGTGT	55.0	50.0	57.7	57.6	982-999 bp	1.0	83.0
rpl20-rps12_p3	CCTCGTTTCACTCTGGTCAT	AGGGGTTTGTACTAGGGTGT	50.0	50.0	57.2	57.6	978-995 bp	1.0	79.4
rpl20-rps12_p4	TCCTCGTTTCACTCTGGTCA	AGAGGGGTTTGTACTAGGGT	50.0	50.0	58.3	57.0	981-998 bp	1.0	78.1
rpl20-rps12_p5	GGATCCTCGTTTCACTCTGG	AGAGGGGTTTGTACTAGGGT	55.0	50.0	57.7	57.0	984-1001 bp	1.0	78.1