Hotspot

trnP-UGG-psaJ

Genome · 61805-62161 · 357 bp

Back to Goniophlebium

Pi 0.171

MarkerSeek score 75.90

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: Genome
length_bp: 357
variable_sites: 61
pi: 0.171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	CCCAAAACAAACACGCTACC	GTGGAAAGGTATATCTTCACA	50.0	38.1	57.9	52.7	424-430 bp	1.0	48.0
trnP-UGG-psaJ_p2	CCAAAACAAACACGCTACCG	GTGGAAAGGTATATCTTCACA	50.0	38.1	58.3	52.7	423-429 bp	1.0	48.0
trnP-UGG-psaJ_p3	CCCAAAACAAACACGCTACC	GGAAAGGTATATCTTCACATATTGCA	50.0	34.6	57.9	57.4	422-428 bp	1.0	48.0
trnP-UGG-psaJ_p4	CCCAAAACAAACACGCTACC	TGGAAAGGTATATCTTCACATATTGC	50.0	34.6	57.9	57.4	423-429 bp	1.0	48.0
trnP-UGG-psaJ_p5	GGATGACGGGATTTGAACCT	GTGGAAAGGTATATCTTCACA	50.0	38.1	57.6	52.7	457-463 bp	1.0	48.0