Hotspot

rps4-trnL-UAA

Genome · 43762-44399 · 638 bp

Back to Goniophlebium

Pi 0.108

MarkerSeek score 81.90

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: Genome
length_bp: 638
variable_sites: 67
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	TCTTTGTTTGGCCTCCAGAC	ATTGCCCGATAAATGGTCCC	50.0	50.0	58.0	58.0	910-925 bp	1.0	87.4
rps4-trnL-UAA_p2	TCTTTGTTTGGCCTCCAGAC	TGTCTCGCTGAGGATAGAGG	50.0	55.0	58.0	58.0	1470-1489 bp	1.0	87.2
rps4-trnL-UAA_p3	TCTTTGTTTGGCCTCCAGAC	CCGTCGAAATGAAGTGGGAT	50.0	50.0	58.0	58.0	1296-1307 bp	1.0	86.8
rps4-trnL-UAA_p4	ATGATCACTAGGCTTTGCGG	ATTGCCCGATAAATGGTCCC	50.0	50.0	58.0	58.0	1367-1382 bp	1.0	85.9
rps4-trnL-UAA_p5	ATCTTTTGGCTTACGGCGAT	ATTGCCCGATAAATGGTCCC	45.0	50.0	58.0	58.0	1168 bp	0.5	59.7