Hotspot

trnT-GGU-trnfM-CAU

Genome · 34408-34890 · 483 bp

Back to Goniophlebium

Pi 0.133
MarkerSeek score 81.70
Primer available yes
Label trnT-GGU-trnfM-CAU

Feature Metadata

label_name
trnT-GGU-trnfM-CAU
feature_type
igs
region
Genome
length_bp
483
variable_sites
63
pi
0.133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnfM-CAU_p1 TTAGCCCCTTGTCGGATTTG CAGGTTCAAATCCTGTCCCC 50.0 55.0 57.9 58.2 573-581 bp 1.0 86.8
trnT-GGU-trnfM-CAU_p2 ACGACTTACGCCTTACCATG CAGGTTCAAATCCTGTCCCC 50.0 55.0 57.7 58.2 548-556 bp 1.0 85.6
trnT-GGU-trnfM-CAU_p3 CGACTTACGCCTTACCATGG CAGGTTCAAATCCTGTCCCC 55.0 55.0 58.4 58.2 547-555 bp 1.0 84.4
trnT-GGU-trnfM-CAU_p4 CGTCACTCTACCCCTGAGTT CAGGTTCAAATCCTGTCCCC 55.0 55.0 58.5 58.2 527-535 bp 1.0 84.3
trnT-GGU-trnfM-CAU_p5 TGATTAGCCCCTTGTCGGAT CAGGTTCAAATCCTGTCCCC 50.0 55.0 58.5 58.2 576-584 bp 1.0 83.9