Hotspot

trnC-GCA-trnG-GCC

Genome · 28707-29206 · 500 bp

Back to Goniophlebium

Pi 0.139
MarkerSeek score 83.20
Primer available yes
Label trnC-GCA-trnG-GCC

Feature Metadata

label_name
trnC-GCA-trnG-GCC
feature_type
igs
region
Genome
length_bp
500
variable_sites
67
pi
0.139
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-trnG-GCC_p1 GATTTGCAGTCCTCCGTCTT GTTCGATTCCCGCTATCCG 50.0 57.9 57.9 58.2 560-561 bp 1.0 80.8
trnC-GCA-trnG-GCC_p2 GATTTGCAGTCCTCCGTCTT GGGTTCGATTCCCGCTATC 50.0 57.9 57.9 57.8 562-563 bp 1.0 80.6
trnC-GCA-trnG-GCC_p3 GATTTGCAGTCCTCCGTCTT GGTTCGATTCCCGCTATCC 50.0 57.9 57.9 57.8 561-562 bp 1.0 80.6
trnC-GCA-trnG-GCC_p4 TCCGTCTTACCGCTTGACTA GTTCGATTCCCGCTATCCG 50.0 57.9 58.2 58.2 548-549 bp 1.0 80.3
trnC-GCA-trnG-GCC_p5 TCCGTCTTACCGCTTGACTA GGTTCGATTCCCGCTATCC 50.0 57.9 58.2 57.8 549-550 bp 1.0 80.1