Hotspot

psbM-petN

Genome · 26894-28078 · 1185 bp

Back to Goniophlebium

Pi 0.136
MarkerSeek score 82.00
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
Genome
length_bp
1185
variable_sites
158
pi
0.136
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 GCCGCGCAGAATAACTAG TTGTAGTTTGGGGACGAAGC 55.6 50.0 56.1 58.1 1219-1232 bp 1.0 57.4
psbM-petN_p2 GCCGCGCAGAATAACTAG CACTTGTAGTTTGGGGACGA 55.6 50.0 56.1 57.5 1222-1235 bp 1.0 54.0
psbM-petN_p3 GCCGCGCAGAATAACTAG TCACTTGTAGTTTGGGGACG 55.6 50.0 56.1 57.5 1223-1236 bp 1.0 54.0
psbM-petN_p4 GCCGCGCAGAATAACTAG ACTTGTAGTTTGGGGACGAAG 55.6 47.6 56.1 57.9 1221-1234 bp 1.0 49.5
psbM-petN_p5 GCCGCGCAGAATAACTAG CACTTGTAGTTTGGGGACGAA 55.6 47.6 56.1 58.2 1222-1235 bp 1.0 49.5