Hotspot

rpoB-trnD-GUC

Genome · 25386-26053 · 668 bp

Back to Goniophlebium

Pi 0.117

MarkerSeek score 86.70

Primer available yes

Label rpoB-trnD-GUC

Feature Metadata

label_name: rpoB-trnD-GUC
feature_type: igs
region: Genome
length_bp: 668
variable_sites: 76
pi: 0.117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnD-GUC_p1	CAAGTTCTGGTAGTACGGGC	GGTGCTCTAACCGATTGAACT	55.0	47.6	58.0	58.0	719-768 bp	1.0	81.7
rpoB-trnD-GUC_p2	GCAAGTTCTGGTAGTACGGG	GGTGCTCTAACCGATTGAACT	55.0	47.6	58.0	58.0	720-769 bp	1.0	81.7
rpoB-trnD-GUC_p3	CAAGTTCTGGTAGTACGGGC	GGATTGACGGGTCTCGAAC	55.0	57.9	58.0	58.0	762-811 bp	1.0	81.1
rpoB-trnD-GUC_p4	GCAAGTTCTGGTAGTACGGG	GGATTGACGGGTCTCGAAC	55.0	57.9	58.0	58.0	763-812 bp	1.0	81.1
rpoB-trnD-GUC_p5	AGTTCTGGTAGTACGGGCAT	GGTGCTCTAACCGATTGAACT	50.0	47.6	58.1	58.0	717-766 bp	1.0	80.6