Hotspot

trnS-GCU-ycf12

Genome · 6721-7279 · 559 bp

Back to Goniophlebium

Pi 0.144

MarkerSeek score 79.40

Primer available yes

Label trnS-GCU-ycf12

Feature Metadata

label_name: trnS-GCU-ycf12
feature_type: igs
region: Genome
length_bp: 559
variable_sites: 80
pi: 0.144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-ycf12_p1	AAAAGGTGTTGGATCCCCAC	CCGAAGTACAAGAGTGACGG	50.0	55.0	58.0	58.0	1613 bp	0.5	59.8
trnS-GCU-ycf12_p2	AAAAGGTGTTGGATCCCCAC	CGAAGTACAAGAGTGACGGG	50.0	55.0	58.0	58.0	1612 bp	0.5	59.8
trnS-GCU-ycf12_p3	ACCCCGGACGTAGAGATTAG	CCGAAGTACAAGAGTGACGG	55.0	55.0	58.0	58.0	1206 bp	0.5	59.7
trnS-GCU-ycf12_p4	ACCCCGGACGTAGAGATTAG	CGAAGTACAAGAGTGACGGG	55.0	55.0	58.0	58.0	1205 bp	0.5	59.7
trnS-GCU-ycf12_p5	AAAAGGTGTTGGATCCCCAC	ACCTACTCCCCATTTTCGGA	50.0	50.0	58.0	58.0	1324 bp	0.5	59.6