Hotspot

psbK-psbI

Genome · 5899-6448 · 550 bp

Back to Goniophlebium

Pi 0.162
MarkerSeek score 82.80
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
Genome
length_bp
550
variable_sites
84
pi
0.162
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 TTGGGTTTCCTGTCAACCAG CTAATCTCTACGTCCGGGGT 50.0 55.0 57.9 58.0 1132 bp 0.5 59.3
psbK-psbI_p2 TGCCAAATTACCCGAAGCTT CTAATCTCTACGTCCGGGGT 45.0 55.0 58.1 58.0 779 bp 0.5 59.2
psbK-psbI_p3 TTGCCAAATTACCCGAAGCT CTAATCTCTACGTCCGGGGT 45.0 55.0 58.1 58.0 780 bp 0.5 59.2
psbK-psbI_p4 TTGGGTTTCCTGTCAACCAG TCGTCCAGGATCGTTCGATA 50.0 50.0 57.9 58.0 1111 bp 0.5 59.1
psbK-psbI_p5 ATCGAGTCTATTGAGGGGCA CTAATCTCTACGTCCGGGGT 50.0 55.0 57.9 58.0 1353 bp 0.5 59.1