Hotspot

ndhJ

LSC · 52055-52594 · 540 bp

Back to Gomphogyne

Pi 0.0021

MarkerSeek score 61.60

Primer available yes

Label ndhJ

Feature Metadata

label_name: ndhJ
feature_type: gene
region: LSC
length_bp: 540
variable_sites: 1
pi: 0.0021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ_p1	AAAGTGAGGATTGGTTTCGT	TGTACAGAATAACAAAGAACGAGT	40.0	33.3	55.2	56.4	598-661 bp	1.0	44.1
ndhJ_p2	AAAAGTGAGGATTGGTTTCGT	TGTACAGAATAACAAAGAACGAGT	38.1	33.3	56.0	56.4	599-662 bp	1.0	44.1
ndhJ_p3	AAAGTGAGGATTGGTTTCGT	ACAGAATAACAAAGAACGAGT	40.0	33.3	55.2	53.1	595-658 bp	1.0	44.1
ndhJ_p4	AAAGTGAGGATTGGTTTCGT	TTGTACAGAATAACAAAGAACGAGT	40.0	32.0	55.2	57.0	599-662 bp	1.0	44.1
ndhJ_p5	AAAAGTGAGGATTGGTTTCGT	ACAGAATAACAAAGAACGAGT	38.1	33.3	56.0	53.1	596-659 bp	1.0	44.1