Hotspot

atpF

LSC · 13002-14351 · 1350 bp

Back to Gomphogyne

Pi 0.00519

MarkerSeek score 61.80

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1350
variable_sites: 7
pi: 0.00519
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTGCTCAATACGTTCACGGA	ACTCTATTTCGAAACTAAATAGGA	45.0	29.2	57.6	53.3	1571-1623 bp	1.0	40.4
atpF_p2	TGCTCAATACGTTCACGGAT	ACTCTATTTCGAAACTAAATAGGA	45.0	29.2	57.3	53.3	1570-1622 bp	1.0	40.4
atpF_p3	TTGCTCAATACGTTCACGGA	ACTCTATTTCGAAACTAAATAGGAA	45.0	28.0	57.6	54.1	1571-1623 bp	1.0	40.4
atpF_p4	TGCTCAATACGTTCACGGAT	ACTCTATTTCGAAACTAAATAGGAA	45.0	28.0	57.3	54.1	1570-1622 bp	1.0	40.4
atpF_p5	TTGCTCAATACGTTCACGGA	ACTCTATTTCGAAACTAAATAGGAAA	45.0	26.9	57.6	54.8	1571-1623 bp	1.0	40.4