Hotspot

ndhF-rpl32

SSC · 115533-116173 · 641 bp

Back to Gomphogyne

Pi 0.00313

MarkerSeek score 60.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 641
variable_sites: 2
pi: 0.00313
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAGTAGAAGTCCCCCTCCT	CTCGACGCCCTTTGTTTTTC	50.0	50.0	57.0	58.0	805-811 bp	1.0	71.7
ndhF-rpl32_p2	AAAGTAGAAGTCCCCCTCCT	TGCTAGCGAAAAGGCTTTCA	50.0	45.0	57.0	58.1	831-837 bp	1.0	71.0
ndhF-rpl32_p3	AAAAGTAGAAGTCCCCCTCCT	CTCGACGCCCTTTGTTTTTC	47.6	50.0	57.7	58.0	806-812 bp	1.0	69.7
ndhF-rpl32_p4	AAAAGTAGAAGTCCCCCTCCT	TGCTAGCGAAAAGGCTTTCA	47.6	45.0	57.7	58.1	832-838 bp	1.0	69.1
ndhF-rpl32_p5	AAAGTAGAAGTCCCCCTCCT	GACGCCCTTTGTTTTTCCAA	50.0	45.0	57.0	57.4	802-808 bp	1.0	67.2