Hotspot

psbB-psbT

LSC · 76876-77067 · 192 bp

Back to Gomphogyne

Pi 0.0426

MarkerSeek score 67.40

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 192
variable_sites: 8
pi: 0.0426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TCCAAAAACTTGGAGATCCTACT	TGGAACTTTAGGTGGTTCGC	39.1	50.0	57.2	58.1	331-335 bp	1.0	51.2
psbB-psbT_p2	TCCAAAAACTTGGAGATCCT	TGGAACTTTAGGTGGTTCGC	40.0	50.0	54.1	58.1	331-335 bp	1.0	51.0
psbB-psbT_p3	TCCTACTACAAGAAGACAGGT	TGGAACTTTAGGTGGTTCGC	42.9	50.0	55.1	58.1	315-319 bp	1.0	50.7
psbB-psbT_p4	TCCAAAAACTTGGAGATCCTACT	GAACTTTAGGTGGTTCGCGA	39.1	50.0	57.2	58.2	329-333 bp	1.0	50.5
psbB-psbT_p5	TCCAAAAACTTGGAGATCCTACT	ACTTTAGGTGGTTCGCGAAA	39.1	45.0	57.2	57.8	327-331 bp	1.0	50.2