Hotspot

psbE-petL

LSC · 67418-68698 · 1281 bp

Back to Gomphogyne

Pi 0.00801

MarkerSeek score 65.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1281
variable_sites: 10
pi: 0.00801
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGTTCAGACCGA	50.0	47.6	57.8	57.5	1393-1396 bp	1.0	69.4
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGTCGTATCTTGTTCAGACCG	50.0	47.6	57.8	57.5	1394-1397 bp	1.0	69.3
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGTTCAGACCGA	55.0	47.6	58.2	57.5	1388-1391 bp	1.0	69.0
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	AGTCGTATCTTGTTCAGACCG	55.0	47.6	58.2	57.5	1389-1392 bp	1.0	69.0
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	GTCGTATCTTGTTCAGACCGA	50.0	47.6	58.4	57.5	1386-1389 bp	1.0	67.8