Hotspot

ndhC-trnV-UAC

LSC · 53786-54493 · 708 bp

Back to Gomphogyne

Pi 0.0539

MarkerSeek score 91.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 708
variable_sites: 35
pi: 0.0539
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 91.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GCCAGAAATGCAAAATGCCA	CCGAGAAAGTCTACGGTTCG	45.0	55.0	58.2	58.1	831-847 bp	1.0	85.0
ndhC-trnV-UAC_p2	GCCAGAAATGCAAAATGCCA	AGTTTGAGCCGTTTTACCGA	45.0	45.0	58.2	57.8	847-863 bp	1.0	83.8
ndhC-trnV-UAC_p3	GAGGGGATAAAACGCCAGAA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.6	58.1	844-860 bp	1.0	83.1
ndhC-trnV-UAC_p4	AGAGGGGATAAAACGCCAGA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.4	58.1	845-861 bp	1.0	83.1
ndhC-trnV-UAC_p5	AAGAGGGGATAAAACGCCAG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.6	58.1	846-862 bp	1.0	83.1