Hotspot

atpH-atpI

LSC · 15043-16103 · 1061 bp

Back to Gomphogyne

Pi 0.0641

MarkerSeek score 90.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1061
variable_sites: 59
pi: 0.0641
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 90.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GATCCCTTCTACAGCTTGGC	TTTTGCAACTTTAGCTGCCG	55.0	45.0	58.0	57.9	1121-1233 bp	1.0	86.6
atpH-atpI_p2	GATCCCTTCTACAGCTTGGC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.0	57.8	1091-1203 bp	1.0	86.4
atpH-atpI_p3	CAGCAGCAATAACAGAAGCG	TTTTGCAACTTTAGCTGCCG	50.0	45.0	57.8	57.9	1044-1156 bp	1.0	85.9
atpH-atpI_p4	CAGCAGCAATAACAGAAGCG	CGAATCCATGGAGGGTCATC	50.0	55.0	57.8	57.8	1014-1126 bp	1.0	85.6
atpH-atpI_p5	CGATCCCTTCTACAGCTTGG	TTTTGCAACTTTAGCTGCCG	55.0	45.0	57.8	57.9	1122-1234 bp	1.0	85.1