Hotspot

trnS-GCU-trnG-UCC

LSC · 8900-9687 · 788 bp

Back to Gomphogyne

Pi 0.00531

MarkerSeek score 66.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 4
pi: 0.00531
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	CATCGTTAGCTTGGAAGGCT	45.0	50.0	58.0	58.0	1540-1578 bp	1.0	83.3
trnS-GCU-trnG-UCC_p2	CACTTCGCTGCATTTCTTGG	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.0	58.0	1897-1935 bp	1.0	82.7
trnS-GCU-trnG-UCC_p3	CTTCGCTGCATTTCTTGGTG	CATCGTTAGCTTGGAAGGCT	50.0	50.0	58.0	58.0	1895-1933 bp	1.0	82.7
trnS-GCU-trnG-UCC_p4	CACTTCGCTGCATTTCTTGG	CGTTAGCTTGGAAGGCTAGG	50.0	55.0	58.0	58.1	1894-1932 bp	1.0	82.5
trnS-GCU-trnG-UCC_p5	CTTCGCTGCATTTCTTGGTG	CGTTAGCTTGGAAGGCTAGG	50.0	55.0	58.0	58.1	1892-1930 bp	1.0	82.5