Hotspot

ycf1

SSC · 123174-126302 · 3129 bp

Back to Goldbachia

Pi 0.0436

MarkerSeek score 65.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 3129
variable_sites: 136
pi: 0.0436
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AATAAGCTGTCCATCGTTTT	ACTGTCTCTTGGTTAGACTACA	35.0	40.9	53.2	55.9	182 bp	1.0	48.0
ycf1_p2	ATAAGCTGTCCATCGTTTTT	ACTGTCTCTTGGTTAGACTACA	35.0	40.9	53.2	55.9	181 bp	1.0	48.0
ycf1_p3	AATAAGCTGTCCATCGTTTTT	ACTGTCTCTTGGTTAGACTACA	33.3	40.9	54.1	55.9	182 bp	1.0	48.0
ycf1_p4	AATAAGCTGTCCATCGTTTT	ACTGTCTCTTGGTTAGACTACAA	35.0	39.1	53.2	56.6	182 bp	1.0	48.0
ycf1_p5	ATAAGCTGTCCATCGTTTTT	ACTGTCTCTTGGTTAGACTACAA	35.0	39.1	53.2	56.6	181 bp	1.0	48.0