Hotspot

ndhE-ndhG

SSC · 117421-117673 · 253 bp

Back to Goldbachia

Pi 0.0558

MarkerSeek score 76.80

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 253
variable_sites: 14
pi: 0.0558
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	TTCGACTCGTGATCAATCCA	TAGGTGCAATTTCTGTGGCT	45.0	45.0	56.7	57.4	357-358 bp	1.0	68.4
ndhE-ndhG_p2	TTCGACTCGTGATCAATCCA	AGGTGCAATTTCTGTGGCTC	45.0	50.0	56.7	58.7	356-357 bp	1.0	67.1
ndhE-ndhG_p3	TCGACTCGTGATCAATCCAT	TAGGTGCAATTTCTGTGGCT	45.0	45.0	56.5	57.4	356-357 bp	1.0	66.5
ndhE-ndhG_p4	TTTCGACTCGTGATCAATCCA	TAGGTGCAATTTCTGTGGCT	42.9	45.0	57.4	57.4	358-359 bp	1.0	66.2
ndhE-ndhG_p5	TCGACTCGTGATCAATCCAT	AGGTGCAATTTCTGTGGCTC	45.0	50.0	56.5	58.7	355-356 bp	1.0	65.2