Hotspot

ndhF-rpl32

SSC · 112133-112979 · 847 bp

Back to Goldbachia

Pi 0.0426
MarkerSeek score 73.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
847
variable_sites
35
pi
0.0426
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGGATGATCCATGAATATTGA ACGATGTCCAATATCCCTTCC 38.1 47.6 52.8 57.2 948-967 bp 1.0 45.1
ndhF-rpl32_p2 GGGATGATCCATGAATATTGA CGATGTCCAATATCCCTTCCT 38.1 47.6 52.8 56.9 947-966 bp 1.0 45.1
ndhF-rpl32_p3 GGGATGATCCATGAATATTGA AACGATGTCCAATATCCCTTCC 38.1 45.5 52.8 57.9 949-968 bp 1.0 45.1
ndhF-rpl32_p4 GGGATGATCCATGAATATTGAT ACGATGTCCAATATCCCTTCC 36.4 47.6 53.5 57.2 948-967 bp 1.0 45.1
ndhF-rpl32_p5 GGGATGATCCATGAATATTGA AAGCTTTCAACGATGTCCAA 38.1 40.0 52.8 55.9 957-976 bp 1.0 45.0