Hotspot

rpl36-rps8

LSC · 79018-79497 · 480 bp

Back to Goldbachia

Pi 0.0426
MarkerSeek score 72.50
Primer available yes
Label rpl36-rps8

Feature Metadata

label_name
rpl36-rps8
feature_type
igs
region
LSC
length_bp
480
variable_sites
20
pi
0.0426
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-rps8_p1 TTTTACGAACGGAAGCCCTT TCGGCGGAGAAATTTTGTGT 45.0 45.0 57.7 58.4 534-540 bp 1.0 79.3
rpl36-rps8_p2 TTTTACGAACGGAAGCCCTT CGGCGGAGAAATTTTGTGTT 45.0 45.0 57.7 57.6 533-539 bp 1.0 79.2
rpl36-rps8_p3 CCCTCCTACGGATTAGTCGA TCGGCGGAGAAATTTTGTGT 55.0 45.0 57.7 58.4 567-573 bp 1.0 79.0
rpl36-rps8_p4 CCCTCCTACGGATTAGTCGA CGGCGGAGAAATTTTGTGTT 55.0 45.0 57.7 57.6 566-572 bp 1.0 78.9
rpl36-rps8_p5 TTTTACGAACGGAAGCCCTT TCCACGAATTTTAGGCGGAA 45.0 45.0 57.7 57.5 620-626 bp 1.0 78.1