Hotspot

trnP-CCA-psaJ

LSC · 65898-66236 · 339 bp

Back to Goldbachia

Pi 0.0299

MarkerSeek score 62.60

Primer available yes

Label trnP-CCA-psaJ

Feature Metadata

label_name: trnP-CCA-psaJ
feature_type: igs
region: LSC
length_bp: 339
variable_sites: 8
pi: 0.0299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-CCA-psaJ_p1	TCCCTCGTTACTGCTCAGAA	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	469-521 bp	1.0	82.7
trnP-CCA-psaJ_p2	ACCCGTGACATTTTGTACCC	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	417-468 bp	1.0	82.7
trnP-CCA-psaJ_p3	CCCGTGACATTTTGTACCCA	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	416-467 bp	1.0	82.7
trnP-CCA-psaJ_p4	ACAGGATTTGAACCCGTGAC	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	428-479 bp	1.0	82.6
trnP-CCA-psaJ_p5	CAGGATTTGAACCCGTGACA	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	427-478 bp	1.0	82.6