Hotspot

trnW-UGG-trnP-CCA

LSC · 65636-65823 · 188 bp

Back to Goldbachia

Pi 0.0428

MarkerSeek score 72.30

Primer available yes

Label trnW-UGG-trnP-CCA

Feature Metadata

label_name: trnW-UGG-trnP-CCA
feature_type: igs
region: LSC
length_bp: 188
variable_sites: 8
pi: 0.0428
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-UGG-trnP-CCA_p1	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	50.0	50.0	58.0	58.1	268-275 bp	1.0	81.6
trnW-UGG-trnP-CCA_p2	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.0	58.1	257-264 bp	1.0	81.6
trnW-UGG-trnP-CCA_p3	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	50.0	50.0	58.0	58.1	255-262 bp	1.0	81.6
trnW-UGG-trnP-CCA_p4	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	50.0	50.0	58.0	58.1	258-265 bp	1.0	81.6
trnW-UGG-trnP-CCA_p5	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	50.0	50.0	58.0	58.1	269-276 bp	1.0	81.5