Hotspot

petA-psbJ

LSC · 62400-63361 · 962 bp

Back to Goldbachia

Pi 0.0513
MarkerSeek score 77.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
962
variable_sites
48
pi
0.0513
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 AGGTTCAATTATCCGAAATGA CAGGATTAGGTTCATCCCTGT 33.3 47.6 52.9 56.8 1003-1014 bp 1.0 45.9
petA-psbJ_p2 AGGTTCAATTATCCGAAATGA TCAGGATTAGGTTCATCCCTGT 33.3 45.5 52.9 58.3 1004-1015 bp 1.0 45.9
petA-psbJ_p3 AAGGTTCAATTATCCGAAATGA CAGGATTAGGTTCATCCCTGT 31.8 47.6 53.8 56.8 1004-1015 bp 1.0 45.9
petA-psbJ_p4 AGGTTCAATTATCCGAAATGAA CAGGATTAGGTTCATCCCTGT 31.8 47.6 53.8 56.8 1003-1014 bp 1.0 45.9
petA-psbJ_p5 AGGTTCAATTATCCGAAATGAA TCAGGATTAGGTTCATCCCTGT 31.8 45.5 53.8 58.3 1004-1015 bp 1.0 45.9