Hotspot

trnL-UUA-trnF-UUC

LSC · 47341-47773 · 433 bp

Back to Goldbachia

Pi 0.115

MarkerSeek score 81.80

Primer available yes

Label trnL-UUA-trnF-UUC

Feature Metadata

label_name: trnL-UUA-trnF-UUC
feature_type: igs
region: LSC
length_bp: 433
variable_sites: 41
pi: 0.115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UUA-trnF-UUC_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	495-523 bp	1.0	86.2
trnL-UUA-trnF-UUC_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	492-520 bp	1.0	83.0
trnL-UUA-trnF-UUC_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	484-512 bp	1.0	82.5
trnL-UUA-trnF-UUC_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	50.0	55.0	57.5	58.6	457-485 bp	1.0	82.4
trnL-UUA-trnF-UUC_p5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	467-495 bp	1.0	82.2