Hotspot

psbZ-trnG-GGC

LSC · 35852-36304 · 453 bp

Back to Goldbachia

Pi 0.0442
MarkerSeek score 72.60
Primer available yes
Label psbZ-trnG-GGC

Feature Metadata

label_name
psbZ-trnG-GGC
feature_type
igs
region
LSC
length_bp
453
variable_sites
20
pi
0.0442
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GGC_p1 TCCTGATGGTTGGTCGAGTA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.8 57.8 603-604 bp 1.0 79.5
psbZ-trnG-GGC_p2 TTTGCGTCTCCTGATGGTTG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.5 57.8 611-612 bp 1.0 77.4
psbZ-trnG-GGC_p3 ATTTGCGTCTCCTGATGGTT CGTCTTCTCCTTGGCAAAGA 45.0 50.0 57.5 57.8 612-613 bp 1.0 77.3
psbZ-trnG-GGC_p4 TCTCCTGATGGTTGGTCGAG CGTCTTCTCCTTGGCAAAGA 55.0 50.0 58.8 57.8 605-606 bp 1.0 74.8
psbZ-trnG-GGC_p5 CCTGATGGTTGGTCGAGTAA CGTCTTCTCCTTGGCAAAGA 50.0 50.0 56.9 57.8 602-603 bp 1.0 72.9