Hotspot

trnE-GAA-trnT-ACC

LSC · 30615-31439 · 825 bp

Back to Goldbachia

Pi 0.0309

MarkerSeek score 72.70

Primer available yes

Label trnE-GAA-trnT-ACC

Feature Metadata

label_name: trnE-GAA-trnT-ACC
feature_type: igs
region: LSC
length_bp: 825
variable_sites: 25
pi: 0.0309
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-GAA-trnT-ACC_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	966-969 bp	1.0	82.0
trnE-GAA-trnT-ACC_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	945-948 bp	1.0	81.6
trnE-GAA-trnT-ACC_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	942-945 bp	1.0	81.4
trnE-GAA-trnT-ACC_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	928-931 bp	1.0	81.3
trnE-GAA-trnT-ACC_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	907-910 bp	1.0	81.2