Hotspot

trnG-GGA-trnR-AGA

LSC · 9391-9554 · 164 bp

Back to Goldbachia

Pi 0.0633
MarkerSeek score 76.20
Primer available yes
Label trnG-GGA-trnR-AGA

Feature Metadata

label_name
trnG-GGA-trnR-AGA
feature_type
igs
region
LSC
length_bp
164
variable_sites
10
pi
0.0633
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GGA-trnR-AGA_p1 AGCCTTCCAAGCTAACGATG TGATATTGCGTCCAATAGGA 50.0 40.0 58.0 53.9 286-288 bp 1.0 51.6
trnG-GGA-trnR-AGA_p2 ACCCTTAGCCTTCCAAGCTA TGATATTGCGTCCAATAGGA 50.0 40.0 58.0 53.9 292-294 bp 1.0 51.5
trnG-GGA-trnR-AGA_p3 AAACCCTTAGCCTTCCAAGC TGATATTGCGTCCAATAGGA 50.0 40.0 58.1 53.9 294-296 bp 1.0 51.1
trnG-GGA-trnR-AGA_p4 AGCCTTCCAAGCTAACGATG TTGATATTGCGTCCAATAGGA 50.0 38.1 58.0 54.8 287-289 bp 1.0 50.6
trnG-GGA-trnR-AGA_p5 ACCCTTAGCCTTCCAAGCTA TTGATATTGCGTCCAATAGGA 50.0 38.1 58.0 54.8 293-295 bp 1.0 50.5