Hotspot

trnA-UGC

IRa · 42898-42932 · 35 bp

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Pi 0.019
MarkerSeek score 52.10
Primer available yes
Label trnA-UGC

Feature Metadata

label_name
trnA-UGC
feature_type
tRNA
region
IRa
length_bp
35
variable_sites
1
pi
0.019
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnA-UGC_p1 TGGAGAGCACAGTACGATGA AAATCCTAGATTCCCGGCCT 50.0 50.0 58.2 58.2 206 bp 1.0 77.6
trnA-UGC_p2 TGGAGAGCACAGTACGATGA AATCCTAGATTCCCGGCCTT 50.0 50.0 58.2 58.2 205 bp 1.0 77.6
trnA-UGC_p3 TGGTAGAATCACTCGGGGAG AAATCCTAGATTCCCGGCCT 55.0 50.0 58.2 58.2 136 bp 1.0 77.4
trnA-UGC_p4 TGGTAGAATCACTCGGGGAG AATCCTAGATTCCCGGCCTT 55.0 50.0 58.2 58.2 135 bp 1.0 77.4
trnA-UGC_p5 TCGTTGGCCTCTATGGTAGA AATCCTAGATTCCCGGCCTT 50.0 50.0 57.6 58.2 148 bp 1.0 75.5
trnA-UGC_p1 AAATCCTAGATTCCCGGCCT TGGAGAGCACAGTACGATGA 50.0 50.0 58.2 58.2 206 bp 1.0 77.6
trnA-UGC_p2 AATCCTAGATTCCCGGCCTT TGGAGAGCACAGTACGATGA 50.0 50.0 58.2 58.2 205 bp 1.0 77.6
trnA-UGC_p3 AAATCCTAGATTCCCGGCCT TGGTAGAATCACTCGGGGAG 50.0 55.0 58.2 58.2 136 bp 1.0 77.4
trnA-UGC_p4 AATCCTAGATTCCCGGCCTT TGGTAGAATCACTCGGGGAG 50.0 55.0 58.2 58.2 135 bp 1.0 77.4
trnA-UGC_p5 AATCCTAGATTCCCGGCCTT TCGTTGGCCTCTATGGTAGA 50.0 50.0 58.2 57.6 148 bp 1.0 75.5