Hotspot

rpl16

LSC · 60427-61608 · 1182 bp

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Pi 0.00113
MarkerSeek score 59.50
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1182
variable_sites
2
pi
0.00113
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 AGTGAAACCCAATTCGGATT AGGGTCTGCTATTTTGTAAACCA 40.0 39.1 55.2 58.0 1326-1327 bp 1.0 40.2
rpl16_p2 CGGAATTCTACTGTGGATCTTCA AGGGTCTGCTATTTTGTAAACCA 43.5 39.1 58.0 58.0 1373-1374 bp 1.0 40.2
rpl16_p3 AGTGAAACCCAATTCGGATT GGGTCTGCTATTTTGTAAACCAG 40.0 43.5 55.2 57.7 1325-1326 bp 1.0 40.2
rpl16_p4 TCGGAATTCTACTGTGGATCT AGGGTCTGCTATTTTGTAAACCA 42.9 39.1 55.9 58.0 1374-1375 bp 1.0 40.2
rpl16_p5 AGTGAAACCCAATTCGGATT GGGTCTGCTATTTTGTAAACCA 40.0 40.9 55.2 56.6 1325-1326 bp 1.0 40.2