Hotspot

trnT-GGU-trnK-UUU

LSC · 4668-5503 · 836 bp

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Pi 0.0865
MarkerSeek score 82.50
Primer available yes
Label trnT-GGU-trnK-UUU

Feature Metadata

label_name
trnT-GGU-trnK-UUU
feature_type
igs
region
LSC
length_bp
836
variable_sites
108
pi
0.0865
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnK-UUU_p1 TACCGCTGAGTTAAAAGGGC AGCCGAGTACTCTACCATTGA 50.0 47.6 57.9 58.0 883-889 bp 1.0 79.7
trnT-GGU-trnK-UUU_p2 TACCGCTGAGTTAAAAGGGC GCCGAGTACTCTACCATTGAG 50.0 52.4 57.9 57.6 882-888 bp 1.0 76.9
trnT-GGU-trnK-UUU_p3 TACCGCTGAGTTAAAAGGGC GCCGAGTACTCTACCATTGA 50.0 50.0 57.9 56.5 882-888 bp 1.0 75.5
trnT-GGU-trnK-UUU_p4 TACCGCTGAGTTAAAAGGGC AGCCGAGTACTCTACCATTG 50.0 50.0 57.9 56.5 883-889 bp 1.0 75.4
trnT-GGU-trnK-UUU_p5 TACCGCTGAGTTAAAAGGGC AAGCCGAGTACTCTACCATTG 50.0 47.6 57.9 57.2 884-890 bp 1.0 73.4