Hotspot

psbD-trnT-GGU

LSC · 4012-4595 · 584 bp

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Pi 0.0251
MarkerSeek score 59.50
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
LSC
length_bp
584
variable_sites
22
pi
0.0251
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 GTCACAAAGGTTGTCCCTGT CGCCAGGAATGAGAAACGTA 50.0 50.0 57.9 58.0 954-956 bp 1.0 82.1
psbD-trnT-GGU_p2 GTCACAAAGGTTGTCCCTGT TACGCCAGGAATGAGAAACG 50.0 50.0 57.9 58.0 956-958 bp 1.0 82.1
psbD-trnT-GGU_p3 ACTAGCTAAGCCGTGGGTAT CGCCAGGAATGAGAAACGTA 50.0 50.0 57.9 58.0 982-984 bp 1.0 81.8
psbD-trnT-GGU_p4 ACAGAGAAGAGCAGCACCTA CGCCAGGAATGAGAAACGTA 50.0 50.0 58.1 58.0 1420-1422 bp 1.0 81.5
psbD-trnT-GGU_p5 ACAGAGAAGAGCAGCACCTA TACGCCAGGAATGAGAAACG 50.0 50.0 58.1 58.0 1422-1424 bp 1.0 81.5