Hotspot

rpl32-ndhF

SSC · 124692-125814 · 1123 bp

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Pi 0.0541
MarkerSeek score 78.20
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
SSC
length_bp
1123
variable_sites
54
pi
0.0541
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 TTTGATATCGAAGTACGTTTTT GCCGTCGGAAAAAGAAGAAG 27.3 50.0 52.2 57.4 1132-1253 bp 1.0 47.6
rpl32-ndhF_p2 TTTTGATATCGAAGTACGTTTT GCCGTCGGAAAAAGAAGAAG 27.3 50.0 52.2 57.4 1133-1254 bp 1.0 47.6
rpl32-ndhF_p3 TTTTTGATATCGAAGTACGTTT GCCGTCGGAAAAAGAAGAAG 27.3 50.0 52.2 57.4 1134-1255 bp 1.0 47.6
rpl32-ndhF_p4 TTTTGATATCGAAGTACGTTTTT GCCGTCGGAAAAAGAAGAAG 26.1 50.0 53.1 57.4 1133-1254 bp 1.0 47.6
rpl32-ndhF_p5 TTTTTGATATCGAAGTACGTTTT GCCGTCGGAAAAAGAAGAAG 26.1 50.0 53.1 57.4 1134-1255 bp 1.0 47.6