Hotspot

trnL-UAG-rpl32

SSC · 123698-124526 · 829 bp

Back to Gnaphalium

Pi 0.0842
MarkerSeek score 77.40
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
829
variable_sites
59
pi
0.0842
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC GGCAGCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.9 837-946 bp 1.0 88.2
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA GGCAGCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.9 838-947 bp 1.0 88.2
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC AAAGGATATTGGGCAGCGTT 50.0 45.0 58.0 57.9 848-957 bp 1.0 88.1
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA AAAGGATATTGGGCAGCGTT 50.0 45.0 58.0 57.9 849-958 bp 1.0 88.1
trnL-UAG-rpl32_p5 TGATCCACAAAGAAGAGCCG GGCAGCGTTAAAAGCTTTGT 50.0 45.0 57.9 57.9 1460-1579 bp 1.0 85.6