Hotspot

ycf4-cemA

LSC · 61206-61666 · 461 bp

Back to Gnaphalium

Pi 0.0535

MarkerSeek score 75.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 461
variable_sites: 23
pi: 0.0535
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	641-666 bp	1.0	86.0
ycf4-cemA_p2	GGGATCGCGGGTCTTTTTAT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	882-907 bp	1.0	84.2
ycf4-cemA_p3	GAGCCATTCCATTGACTCGT	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.0	58.0	992-1017 bp	1.0	83.9
ycf4-cemA_p4	GGGATCGCGGGTCTTTTTAT	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.0	58.0	1233-1258 bp	1.0	83.0
ycf4-cemA_p5	CGTTGGGGATTTCCTGGAAA	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.1	58.0	1125-1150 bp	1.0	83.0