Hotspot

ndhC-trnV-UAC

LSC · 50952-52148 · 1197 bp

Back to Gnaphalium

Pi 0.0262

MarkerSeek score 74.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1197
variable_sites: 30
pi: 0.0262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTTGCTACTCGGAGCTA	GAGAAGGTCTACGGTTCGAG	50.0	55.0	57.9	57.2	1337-1342 bp	1.0	77.1
ndhC-trnV-UAC_p2	TCCTTTGCTACTCGGAGCTA	CGAGAAGGTCTACGGTTCGA	50.0	55.0	57.9	58.9	1338-1343 bp	1.0	76.4
ndhC-trnV-UAC_p3	ACTCGGAGCTAAAAATCCCG	GAGAAGGTCTACGGTTCGAG	50.0	55.0	57.7	57.2	1328-1333 bp	1.0	75.8
ndhC-trnV-UAC_p4	TTCTCCGGTCCTTTGCTACT	GAGAAGGTCTACGGTTCGAG	50.0	55.0	58.4	57.2	1345-1350 bp	1.0	75.3
ndhC-trnV-UAC_p5	ACTCGGAGCTAAAAATCCCG	CGAGAAGGTCTACGGTTCGA	50.0	55.0	57.7	58.9	1329-1334 bp	1.0	75.0