Hotspot

ycf3-trnS-GGA

LSC · 44720-45690 · 971 bp

Back to Gnaphalium

Pi 0.0265
MarkerSeek score 74.60
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
971
variable_sites
25
pi
0.0265
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTCTCCTGAAGTTGTCGGAA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 1077-1105 bp 1.0 69.7
ycf3-trnS-GGA_p2 TTTCTCCTGAAGTTGTCGGA TTCCAATGCTACGCCTTCAA 45.0 45.0 56.4 57.8 1078-1106 bp 1.0 69.7
ycf3-trnS-GGA_p3 TTCTCCTGAAGTTGTCGGAA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1115-1143 bp 1.0 69.3
ycf3-trnS-GGA_p4 TTTCTCCTGAAGTTGTCGGA GGATTCGAACCCTCGGTAAG 45.0 55.0 56.4 57.8 1116-1144 bp 1.0 69.3
ycf3-trnS-GGA_p5 TTCTCCTGAAGTTGTCGGAA AGTTCCAATGCTACGCCTTC 45.0 50.0 56.4 58.3 1079-1107 bp 1.0 69.1