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psbZ-trnG-GCC

LSC · 36231-36554 · 324 bp

Back to Gnaphalium

Pi 0.0334

MarkerSeek score 66.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 324
variable_sites: 10
pi: 0.0334
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	GCGTCTTCTCCTTGGCAAG	50.0	57.9	57.8	58.8	461-482 bp	1.0	69.4
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTC	GCGTCTTCTCCTTGGCAAG	55.0	57.9	58.3	58.8	459-480 bp	1.0	69.0
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	GAATCGAACCCGCGTCTTC	50.0	57.9	57.8	59.0	472-493 bp	1.0	68.0
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	GAATCGAACCCGCGTCTTC	55.0	57.9	58.3	59.0	470-491 bp	1.0	67.6
psbZ-trnG-GCC_p5	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCGTCTTC	50.0	55.6	57.8	57.8	471-492 bp	1.0	66.6