Hotspot

psbC-trnS-UGA

LSC · 35370-35599 · 230 bp

Back to Gnaphalium

Pi 0.0405

MarkerSeek score 74.70

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 230
variable_sites: 9
pi: 0.0405
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	334-342 bp	1.0	82.6
psbC-trnS-UGA_p2	TCTTCGTAGGGCATTTGTGG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	57.9	58.0	417-425 bp	1.0	82.2
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	58.2	58.2	275-283 bp	1.0	81.9
psbC-trnS-UGA_p4	TCTTCGTAGGGCATTTGTGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.9	58.2	358-366 bp	1.0	81.2
psbC-trnS-UGA_p5	AGCTGCAGCAGGATTTGAAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	58.4	58.0	377-385 bp	1.0	80.4