Hotspot

trnT-GGU-psbD

LSC · 31597-32938 · 1342 bp

Back to Gnaphalium

Pi 0.0336
MarkerSeek score 75.40
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1342
variable_sites
42
pi
0.0336
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGACCCGTTCAGGAATTCA GAAACGGTCCCTACGTAACC 50.0 55.0 57.8 58.0 1477-1523 bp 1.0 84.8
trnT-GGU-psbD_p2 CAGTGACCCGTTCAGGAATT GAAACGGTCCCTACGTAACC 50.0 55.0 57.8 58.0 1479-1525 bp 1.0 84.7
trnT-GGU-psbD_p3 CGTAAGCCCTCGGTTCAAAT GAAACGGTCCCTACGTAACC 50.0 55.0 58.3 58.0 1413-1465 bp 1.0 84.2
trnT-GGU-psbD_p4 TACAGTGACCCGTTCAGGAA GAAACGGTCCCTACGTAACC 50.0 55.0 58.3 58.0 1481-1527 bp 1.0 83.9
trnT-GGU-psbD_p5 TAAGCCCTCGGTTCAAATCC GAAACGGTCCCTACGTAACC 50.0 55.0 57.6 58.0 1411-1463 bp 1.0 83.0