Hotspot

trnG-UCC-trnT-GGU

LSC · 31152-31536 · 385 bp

Back to Gnaphalium

Pi 0.1

MarkerSeek score 74.20

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 385
variable_sites: 27
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	AATCAAACCGAAAGACCCCT	ATTTGAACCGAGGGCTTACG	45.0	50.0	57.0	58.3	393-503 bp	1.0	78.9
trnG-UCC-trnT-GGU_p2	ATCAAACCGAAAGACCCCTT	ATTTGAACCGAGGGCTTACG	45.0	50.0	57.0	58.3	392-502 bp	1.0	78.9
trnG-UCC-trnT-GGU_p3	ATCAAACCGAAAGACCCCTT	AGGGCTTACGCTTTACACTC	45.0	50.0	57.0	57.6	382-492 bp	1.0	78.1
trnG-UCC-trnT-GGU_p4	ATCAAACCGAAAGACCCCTT	GAGGGCTTACGCTTTACACT	45.0	50.0	57.0	57.6	383-493 bp	1.0	78.1
trnG-UCC-trnT-GGU_p5	ATCAAACCGAAAGACCCCTT	GGGCTTACGCTTTACACTCT	45.0	50.0	57.0	57.6	381-491 bp	1.0	78.1