Hotspot

trnY-GUA-trnE-UUC

LSC · 12003-12185 · 183 bp

Back to Gnaphalium

Pi 0.0393
MarkerSeek score 74.80
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
183
variable_sites
7
pi
0.0393
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 CGAATTTACAGTCCGTCCCC AAAACGAGGGTTCAAGTCCC 55.0 50.0 58.4 58.0 251-256 bp 1.0 80.8
trnY-GUA-trnE-UUC_p2 ACGAATTTACAGTCCGTCCC AAAACGAGGGTTCAAGTCCC 50.0 50.0 57.6 58.0 252-257 bp 1.0 80.6
trnY-GUA-trnE-UUC_p3 CGAATTTACAGTCCGTCCCC CCCCTATGGTCCAGTGGTTA 55.0 55.0 58.4 58.1 292-297 bp 1.0 79.6
trnY-GUA-trnE-UUC_p4 CGAATTTACAGTCCGTCCCC GATGTATGATGGCCACAGGG 55.0 55.0 58.4 58.1 320 bp 0.5 56.4
trnY-GUA-trnE-UUC_p5 ACGAATTTACAGTCCGTCCC GATGTATGATGGCCACAGGG 50.0 55.0 57.6 58.1 321 bp 0.5 56.2