Hotspot

petN-psbM

LSC · 10442-10976 · 535 bp

Back to Gnaphalium

Pi 0.0566

MarkerSeek score 77.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 535
variable_sites: 29
pi: 0.0566
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	GGAGGGACAGAGAGCAATTG	50.0	55.0	58.2	58.0	1324-1338 bp	1.0	83.3
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	AAGGAGGGACAGAGAGCAAT	50.0	50.0	58.2	58.0	1326-1340 bp	1.0	83.2
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	AGGAGGGACAGAGAGCAATT	50.0	50.0	58.2	58.0	1325-1339 bp	1.0	83.2
petN-psbM_p4	TCGGGAAAGGGTACGGATTA	GGAGGGACAGAGAGCAATTG	50.0	55.0	57.8	58.0	1695 bp	0.5	58.5
petN-psbM_p5	TCGGGAAAGGGTACGGATTA	AGGAGGGACAGAGAGCAATT	50.0	50.0	57.8	58.0	1696 bp	0.5	58.5