Hotspot

rrn23

IRa · 131659-134467 · 2809 bp

Back to Gmelina

Pi 0.0142

MarkerSeek score 46.30

Primer available yes

Label rrn23

Feature Metadata

label_name: rrn23
feature_type: rRNA
region: IRa
length_bp: 2809
variable_sites: 80
pi: 0.0142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23_p1	AAACTTCTGTCGCTCCATCC	TCCATCCATTTAGCAGCACC	50.0	50.0	57.9	57.9	2985-2994 bp	1.0	81.1
rrn23_p2	AAACTTCTGTCGCTCCATCC	CCATCCATTTAGCAGCACCT	50.0	50.0	57.9	57.9	2984-2993 bp	1.0	81.1
rrn23_p3	AAACTTCTGTCGCTCCATCC	GCACCTTAGGATGGCATAGC	50.0	55.0	57.9	58.5	2970-2979 bp	1.0	77.8
rrn23_p4	TCAAAACTTCTGTCGCTCCA	TCCATCCATTTAGCAGCACC	45.0	50.0	57.4	57.9	2988-2997 bp	1.0	77.0
rrn23_p5	TCAAAACTTCTGTCGCTCCA	CCATCCATTTAGCAGCACCT	45.0	50.0	57.4	57.9	2987-2996 bp	1.0	77.0