Hotspot

accD-psaI

LSC · 60061-60757 · 697 bp

Back to Gmelina

Pi 0.00765

MarkerSeek score 64.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 697
variable_sites: 10
pi: 0.00765
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCGTACCGCGCAATCTTTTA	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	856-884 bp	1.0	80.8
accD-psaI_p2	TCGTACCGCGCAATCTTTTA	AAGAAGCCATTGCAATTGCC	45.0	45.0	58.0	57.9	862-890 bp	1.0	80.1
accD-psaI_p3	TAAGCTCCACGCCTTCTTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	813-841 bp	1.0	80.1
accD-psaI_p4	CTCCACGCCTTCTTTCCTTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	809-837 bp	1.0	80.0
accD-psaI_p5	TAAGCTCCACGCCTTCTTTC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.9	57.9	819-847 bp	1.0	79.5