Hotspot

ndhC-trnV-UAC

LSC · 51621-52624 · 1004 bp

Back to Gmelina

Pi 0.00747

MarkerSeek score 61.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1004
variable_sites: 14
pi: 0.00747
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAATCCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1130-1135 bp	1.0	79.4
ndhC-trnV-UAC_p2	CTCCGGCCTTTTCCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	1149-1154 bp	1.0	76.7
ndhC-trnV-UAC_p3	GGGGCTAAAATCCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1129-1134 bp	1.0	76.6
ndhC-trnV-UAC_p4	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1118-1123 bp	1.0	75.5
ndhC-trnV-UAC_p5	GGGGCTAAAATCCCGGAAAT	AAGGTCTACGGTTCGAGTCC	50.0	55.0	57.9	58.8	1128-1133 bp	1.0	74.3