Hotspot

petN-psbM

LSC · 29694-30705 · 1012 bp

Back to Gmelina

Pi 0.00346

MarkerSeek score 62.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1012
variable_sites: 7
pi: 0.00346
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TCTTGCGTTTATTGCTACTGC	50.0	42.9	58.0	57.3	1106-1134 bp	1.0	68.6
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TCTTGCGTTTATTGCTACTGC	50.0	42.9	58.0	57.3	1104-1132 bp	1.0	68.6
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	TCTTGCGTTTATTGCTACTGC	55.0	42.9	57.9	57.3	1103-1131 bp	1.0	68.0
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TCTTGCGTTTATTGCTACTGC	50.0	42.9	58.2	57.3	1158-1186 bp	1.0	67.3
petN-psbM_p5	TCGTAGTGTGGGGAAGAAGT	TTCTTGCGTTTATTGCTACTGC	50.0	40.9	58.0	57.9	1105-1133 bp	1.0	65.9