Hotspot

trnC-GCA-petN

LSC · 28758-29603 · 846 bp

Back to Gmelina

Pi 0.00591

MarkerSeek score 65.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 10
pi: 0.00591
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	941-956 bp	1.0	81.7
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	939-954 bp	1.0	81.7
trnC-GCA-petN_p3	ATCCGGGAAAATTTGCACCT	ACTTCTTCCCCACACTACGA	45.0	50.0	58.1	58.0	1018-1033 bp	1.0	81.6
trnC-GCA-petN_p4	ATCCGGGAAAATTTGCACCT	TTCTTCCCCACACTACGAGT	45.0	50.0	58.1	58.0	1016-1031 bp	1.0	81.6
trnC-GCA-petN_p5	ATCCGGGAAAATTTGCACCT	CACTTCTTCCCCACACTACG	45.0	55.0	58.1	57.9	1019-1034 bp	1.0	81.0