Hotspot

rps16-trnQ-UUG

LSC · 6297-7475 · 1179 bp

Back to Gmelina

Pi 0.00891

MarkerSeek score 63.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1179
variable_sites: 21
pi: 0.00891
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAAATTGAAATGGGGCGTGG	50.0	50.0	57.9	58.0	1321-1361 bp	1.0	81.0
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1304-1344 bp	1.0	80.5
rps16-trnQ-UUG_p3	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1263-1303 bp	1.0	80.5
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1246-1286 bp	1.0	80.0
rps16-trnQ-UUG_p5	CAACGGATCGTGTCCTTCAA	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.2	58.0	1322-1362 bp	1.0	79.9