Hotspot

trnH-GUG-psbA

LSC · 76-396 · 321 bp

Back to Gmelina

Pi 0.0254
MarkerSeek score 62.70
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
321
variable_sites
16
pi
0.0254
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 430-452 bp 1.0 84.6
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG GAGTGGGAGCTTAGTTTCCG 55.0 55.0 58.1 58.0 1010-1032 bp 1.0 81.5
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA GAGTGGGAGCTTAGTTTCCG 50.0 55.0 57.9 58.0 1024-1046 bp 1.0 81.5
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC GAGTGGGAGCTTAGTTTCCG 50.0 55.0 57.9 58.0 1023-1045 bp 1.0 81.5
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC GAGTGGGAGCTTAGTTTCCG 50.0 55.0 57.9 58.0 1025-1047 bp 1.0 81.5